Polyacrylamide Gel Electrophoresis of Humic and Fulvic Acids after Acid Hydrolysis
نویسندگان
چکیده
Two soil humic acids (HA), a marine sediment HA, and a soil fulvic acid (FA), were fractionated by polyacrylamide gel electrophoresis in the presence of denaturing agents before and after 6N HCl hydrolysis. After acid hydrolysis, the intensity of the high molecular size (MS) fraction decreased considerably in all HAs. On the other hand, a new high-MS fraction appeared in the FA after hydrolysis. The electrophoretic data indicate that acid hydrolysis produced a transformation in the humic macromolecule, inducing either depolymerization or condensation, depending on the nature of the humic fraction. Therefore, the advantages and disadvantages of using acid hydrolysis should be carefully considered before this treatment is carried out.
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